2d tissue culture plates human aortic ecs  (PromoCell)

Journal: Journal of tissue engineering and regenerative medicine

Article Title: 3D matrix embedding inhibits cycloheximide-mediated sensitization to TNF-alpha-induced apoptosis of human endothelial cells

doi: 10.1002/term.2609

Figure Lengend Snippet: Human aortic ECs were cultured either on conventional 2D-polystyrene tissue culture plates (TCPS) or within 3D Gelfoam matrices (GF). A CHX-mediated sensitization to TNF-α was used to induce EC apoptosis. Results are given as the mean SD of n = 8 independent experiments. **p < 0.01.

Article Snippet: 2.1 Cell culture on 3D-matrices and on 2D-tissue culture plates Human aortic ECs were obtained from Promocell (Heidelberg, Germany) and grown in optimized endothelial growth medium-2 (Promocell) supplemented with 5% fetal bovine serum either on polystyrene-coated tissue culture plates or embedded within 3D matrices of denaturated collagen (Gelfoam; Pfizer, New York, USA) as previously published ( Methe et al., 2005 ; Nugent & Edelman, 2001 ): compressed sponges were cut into 1 × 1 × 0.3 cm blocks and hydrated in culture medium at 37 °C for ≥4 h. Then 4.5 × 10 4 ECs (suspended in ~50 μL media) were seeded onto one surface of the hydrated matrix, allowed 1.5 h to attach before turning the matrix over and seeding an additional 4.5 × 10 4 in growth media.

Techniques: Cell Culture

Journal: Journal of tissue engineering and regenerative medicine

Article Title: 3D matrix embedding inhibits cycloheximide-mediated sensitization to TNF-alpha-induced apoptosis of human endothelial cells

doi: 10.1002/term.2609

Figure Lengend Snippet: ECs were either cultured on conventional 2D-polystyrene tissue culture plates with (TCPS) or without (CRTL) addition of TNF-α and CHX or within a Gelfoam matrix with addition of TNF-α and CHX. Results show the mean ± SD of n = 8 independent Western blots without induction of EC-apoptosis (Figure 3C), after 15 minutes (Figure 3A) and 1 hour (Figure 3B) of stimulation TNF-α and CHX. *p < 0.05; **p < 0.01.

Article Snippet: 2.1 Cell culture on 3D-matrices and on 2D-tissue culture plates Human aortic ECs were obtained from Promocell (Heidelberg, Germany) and grown in optimized endothelial growth medium-2 (Promocell) supplemented with 5% fetal bovine serum either on polystyrene-coated tissue culture plates or embedded within 3D matrices of denaturated collagen (Gelfoam; Pfizer, New York, USA) as previously published ( Methe et al., 2005 ; Nugent & Edelman, 2001 ): compressed sponges were cut into 1 × 1 × 0.3 cm blocks and hydrated in culture medium at 37 °C for ≥4 h. Then 4.5 × 10 4 ECs (suspended in ~50 μL media) were seeded onto one surface of the hydrated matrix, allowed 1.5 h to attach before turning the matrix over and seeding an additional 4.5 × 10 4 in growth media.

Techniques: Cell Culture, Western Blot

Journal: Journal of tissue engineering and regenerative medicine

Article Title: 3D matrix embedding inhibits cycloheximide-mediated sensitization to TNF-alpha-induced apoptosis of human endothelial cells

doi: 10.1002/term.2609

Figure Lengend Snippet: Figure 6A shows phosphorylation of p38-MAPK at Thr180/Tyr182 after treatment with a combination of TNF-α and CHX for 10 minutes for ECs on 2D-TCPS and in a Gelfoam matrix (GF). An unstimulated 2D-control culture (CRTL) without addition of cytokines was evaluated. Figure 6B shows basal protein expression of p38-MAPK as determined by western blot for culture of ECs on conventional 2D-polystyrene tissue culture plates (TCPS) and in a 3D Gelfoam matrix (GF). For evaluation of basal expression no cytokines were added. Results show the mean ± SD of n = 8 independent western blots. *p < 0.05; **p < 0.01; ns = not significant.

Article Snippet: 2.1 Cell culture on 3D-matrices and on 2D-tissue culture plates Human aortic ECs were obtained from Promocell (Heidelberg, Germany) and grown in optimized endothelial growth medium-2 (Promocell) supplemented with 5% fetal bovine serum either on polystyrene-coated tissue culture plates or embedded within 3D matrices of denaturated collagen (Gelfoam; Pfizer, New York, USA) as previously published ( Methe et al., 2005 ; Nugent & Edelman, 2001 ): compressed sponges were cut into 1 × 1 × 0.3 cm blocks and hydrated in culture medium at 37 °C for ≥4 h. Then 4.5 × 10 4 ECs (suspended in ~50 μL media) were seeded onto one surface of the hydrated matrix, allowed 1.5 h to attach before turning the matrix over and seeding an additional 4.5 × 10 4 in growth media.

Techniques: Expressing, Western Blot

Journal: Journal of tissue engineering and regenerative medicine

Article Title: 3D matrix embedding inhibits cycloheximide-mediated sensitization to TNF-alpha-induced apoptosis of human endothelial cells

doi: 10.1002/term.2609

Figure Lengend Snippet: Figure 8A shows phosphorylation of FAK at Tyr397 as determined by Western Blot (n=8). ECs cultured on conventional 2D-tissue culture plates (TCPS) or in a 3D Gelfoam matrix (GF) were stimulated with a combination of TNF-α and CHX for 10 minutes. An unstimulated culture of ECs on conventional 2D-polystyrene culture plates served as control (CRTL). Figure 8B shows basal protein expression of FAK as determined by Western blot, comparing culture of ECs on 2D-TCPS and in a Gelfoam matrix (GF). When comparing basal protein expression no cytokines were added. Results show the mean ± SD of n = 8 independent western blots. *p < 0.05; **p < 0.01; ns = not significant.

Article Snippet: 2.1 Cell culture on 3D-matrices and on 2D-tissue culture plates Human aortic ECs were obtained from Promocell (Heidelberg, Germany) and grown in optimized endothelial growth medium-2 (Promocell) supplemented with 5% fetal bovine serum either on polystyrene-coated tissue culture plates or embedded within 3D matrices of denaturated collagen (Gelfoam; Pfizer, New York, USA) as previously published ( Methe et al., 2005 ; Nugent & Edelman, 2001 ): compressed sponges were cut into 1 × 1 × 0.3 cm blocks and hydrated in culture medium at 37 °C for ≥4 h. Then 4.5 × 10 4 ECs (suspended in ~50 μL media) were seeded onto one surface of the hydrated matrix, allowed 1.5 h to attach before turning the matrix over and seeding an additional 4.5 × 10 4 in growth media.

Techniques: Western Blot, Cell Culture, Expressing

Journal: Journal of tissue engineering and regenerative medicine

Article Title: 3D matrix embedding inhibits cycloheximide-mediated sensitization to TNF-alpha-induced apoptosis of human endothelial cells

doi: 10.1002/term.2609

Figure Lengend Snippet: Association of RIP with FAK were analyzed by Western Blot using anti-RIP-IgG-antibodies was compared in ECs cultured on TCPS with ECs cultured within a 3D Gelfoam matrix (GF) after addition of TNF-α and CHX. Additionally, both conditions were compared to an unstimulated control 2D-culture on TCPS (CRTL) without addition of cytokines. Results show the mean ± SD of n = 8 independent western blots. **p < 0.01; ns = not significant.

Article Snippet: 2.1 Cell culture on 3D-matrices and on 2D-tissue culture plates Human aortic ECs were obtained from Promocell (Heidelberg, Germany) and grown in optimized endothelial growth medium-2 (Promocell) supplemented with 5% fetal bovine serum either on polystyrene-coated tissue culture plates or embedded within 3D matrices of denaturated collagen (Gelfoam; Pfizer, New York, USA) as previously published ( Methe et al., 2005 ; Nugent & Edelman, 2001 ): compressed sponges were cut into 1 × 1 × 0.3 cm blocks and hydrated in culture medium at 37 °C for ≥4 h. Then 4.5 × 10 4 ECs (suspended in ~50 μL media) were seeded onto one surface of the hydrated matrix, allowed 1.5 h to attach before turning the matrix over and seeding an additional 4.5 × 10 4 in growth media.

Techniques: Western Blot, Cell Culture

Journal: Pharmaceuticals

Article Title: Synthesis of Nitrostyrylthiazolidine-2,4-dione Derivatives Displaying Antileishmanial Potential

doi: 10.3390/ph17070878

Figure Lengend Snippet: Results of the in vitro evaluations (activity and cytotoxicity on HepG2) of the 21 compounds presenting both good solubility in the culture medium (Schneider’s Drosophila Medium) and antileishmanial EC 50 activity values < 30 µM.

Article Snippet: EC 50 values were calculated by non-linear regression analysis processed dose-response curves, using TableCurve 2D V5.0 software (Systat Software Inc. (Palo Alto, CA, USA)).

Techniques: In Vitro, Activity Assay, Solubility

Journal: Pharmaceuticals

Article Title: Synthesis of Nitrostyrylthiazolidine-2,4-dione Derivatives Displaying Antileishmanial Potential

doi: 10.3390/ph17070878

Figure Lengend Snippet: Sensitivity of wild-type and NTR-overexpressing L. donovani promastigotes to compound 14c .

Article Snippet: EC 50 values were calculated by non-linear regression analysis processed dose-response curves, using TableCurve 2D V5.0 software (Systat Software Inc. (Palo Alto, CA, USA)).

Techniques: